For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules. The volume of gas in the test tube to start with is slightly affected by the amount which the bung is pushed down each time, if the bung is pushed down further then the volume in the tube will be less so the 30cm3 of gas is reached faster.
Prepare 6 different concentrations of hydrogen peroxide in gradual increments, taking the standard low concentration of hydrogen peroxide used in the pilot study as defined in STEP B, POINT 1 as the lowest concentration in the gradually increasing scale.
Hydrogen peroxide as a metabolic by-product in the cells. So in cm3, the actual volume could have been either Doing this could lead to being disqualified from all the subjects that you are taking. Students may notice the heat if they put their hands on the conical flask. Each of us is qualified to a high level in our area of expertise, and we can write you a fully researched, fully referenced complete original answer to your essay question.
Be sure to clamp the gas syringe securely but carefully. Push the plunger on the syringe and immediately start the stopclock. Increasing the Hydrogen Peroxide Concentration after the point of saturation has been reached will not cause the rate of reaction to go up any more.
This should tell me whether the reaction is indeed a first-order reaction, or if a curve of best fit is required. This means that having the same surface area of yeast in each reaction is very important in ensuring a fair test because the number of molecules exposed to collisions must be the same.
Rinse splashes of peroxide and pureed potato off the skin as quickly as possible. I did this by only using pipettes, which have a very small apparatus error when compared to beakers.
If the line of best fit is correct, it would make this value an anomaly, which clearly it is not since it is the most accurate value on the graph.
It is a very important enzyme in reproductive reactions. Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide.
In the liver, Hydrogen peroxide is a toxic by-product of fatty acid oxidation. This means that in my results, the volume of gas produced in the first 5 seconds may have been higher than it should have been if I had used exactly 0.
It is one of the fastest acting enzymes it needs to maintain low levels of hydrogen peroxide. However, the hypothesized graph will have a concave down as seen in figure 2. If you like this post please consider linking to it using this code: This may have been because I only used a pipette when measuring the hydrogen peroxide, and poured the water into the volumetric flask to make up the rest of the cm3.
You have been warned. I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order to ensure a fair test.
Catalase binds with the substrate molecules though their active sites and catalyses by processing them to form the ultimate by-products of water and oxygen, at a rapid rate as it has high turnover numbers.
Below is a summary of all the percentage errors. Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of molecules of hydrogen peroxide to water and oxygen each second.
The optimum temperature also varies by species. This could have affected my results for several reasons. Extracts of this project must not be included in any projects that you submit for marking. Role of hydrogen peroxide in the cytotoxicity.
After reaching maximum velocity V maxa plateau region is observed that shows no further increment in the rate of reaction as all of the enzyme molecules are already bounded with substrate molecules.
However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration.
The diagram below shows what happens. Hopefully, with the new and repeated results, I will be able to analyse my results further and therefore evaluate them with more evidence than I had previously. Keep in brown bottles because hydrogen peroxide degrades faster in the light. Measure another 20 cm3 pureed potato into it.
In this procedure, 2 cm3 of 10 vol hydrogen peroxide will release 20 cm3 of oxygen if the reaction goes to completion. This is shown when oxygen stops being produced and the same results are recorded five times.
The time taken to fit into and leave the active site is the limiting factor in the rate of reaction.
As hydrogen peroxide is active and harmful to cells and tissues of organisms, its decomposition therefore needs to be speeded up greatly in order to prevent it from intoxication in the cell.The amount of hydrogen peroxide decreases and the amount of oxygen gas formation increases. What is the purpose of preparing a tube of hydrogen peroxide.
Investigation - Hydrogen Peroxide An investigation into how the concentration of the substrate 'hydrogen peroxide' affects the rate at which it is broken down to oxygen and water by the enzyme catalase. Hydrogen peroxide is released due to many metabolic reactions in the body, which gives toxicity to the living cells.
Hence, there is a chemical.
An Investigation of Catalase/Hydrogen Peroxide Enzymatic Kinetics Lauren Perryman With Racquel Robinson, Kimberly Roberts, Halie Choate pH, inhibitors, substrate concentrations, and more can all affect the kinetics of an enzyme. Enzymatic kinetic studies shed light on the biological have a considerable effect on the rate of the.
This investigation looks at the rate of oxygen production by the catalase in pureed potato as the concentration of hydrogen peroxide varies. The oxygen produced in 30 seconds is collected over water. The oxygen produced in 30 seconds is collected over water. 4. Repeat the experiment with hydrogen peroxide concentrations of 16%, 12%, 10%, 8%, 4% and 0%.
The 0% concentration of hydrogen peroxide solution is done as a control solution to show that at 0% concentration no reaction occurs.
The factors affecting rate of decomposition are temperature, pH and changes, concentration of hydrogen peroxide and concentration of catalase. As this investigation investigates the effect of concentration of catalase on the rate of decomposition of hydrogen peroxide, the temperature, pH and concentration of hydrogen peroxide are .Download